The District Municipality of Muskoka, more generally referred to as the District of Muskoka or Muskoka, is a regional municipality in Central Ontario, Canada. Muskoka extends from Georgian Bay in the west, to the northern tip of Lake Couchiching in the south, to the western border of Algonquin Provincial Park in the east. A two-hour drive north of Toronto, Muskoka spans 6,475 km2 (2,500 sq mi). Muskoka has some 1,600 lakes, making it a popular cottaging destination.

This region, which, along with Haliburton, Kawartha Lakes, and Peterborough County is referred to as "cottage country", sees over 2.1 million visitors annually. Muskoka is an area populated with several villages and towns, farming communities, and lakeside vacation hotels and resorts near to golf courses, country clubs, and marinas. The regional government seat is Bracebridge and the largest population centre is Huntsville.

Muskoka is geographically located within the Central Ontario region of the province, although it is treated as part of Northern Ontario by some government programs.

Muskoka is a summer destination for Toronto residents and was the #1 most searched Canadian destination for vacation rentals in 2017. The Muskoka region was also ranked #1 for best trips of 2011 by National Geographic, and was a finalist for the same distinction in 2012.

The name of the municipality derives from a First Nations chief of the 1850s. Lake Muskoka was then the hunting grounds of a troop led by Chief Yellowhead or Mesqua Ukie or Musquakie. He was revered by the government, who built a home for him in Orillia where he lived until his death at the age of 95.

Muskoka has 60,000 permanent residents, but an additional 100,000 seasonal property owners spend their summers in the region every year, making this a major summer colony. Due to the regions' popularity and high property costs, hundreds of Muskoka properties are available to rent short-term through platforms.